Stable Nitrogen Isotope Analysis of Amino Acids by Using Gas Chromatography/Isotope Ratio Mass Spectrometry (GC/IRMS) System with High-temperature Combustion Interface
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چکیده
منابع مشابه
δ13C analysis of amino acids in human hair using trimethylsilyl derivatives and gas chromatography/combustion/isotope ratio mass spectrometry.
RATIONALE To provide a simple one-step derivatization procedure for the analysis of a wide variety of amino acids in human hair by gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS). N,O-Bis(trimethylsilyl)trifluoroacetamide (BSTFA) derivatization is already widely used outside the IRMS community, is applicable to a variety of functional groups, and provides products that...
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RATIONALE: Organic nitrogen (N) greatly exceeds inorganic N in soils, but the complexity and heterogeneity of this important soil N pool make investigations into the fate of N-containing additions and soil organic N cycling challenging. This paper details a novel approach to investigate the fate of applied N in soils, generating quantitative measures of microbial assimilation and of newly synth...
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The stable carbon isotope compositions of tetrols, erythritol and threitol were determined by gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS). Using four tetrols with various delta(13)C values derivatized by methylboronic acid, the carbon isotope analysis method achieved excellent reproducibility and high accuracy. There was no carbon isotopic fractionation during the ...
متن کاملIsotope-Ratio-Monitoring Gas Chromatography-Mass Spectrometry
RECEIVED for review February 21, 1978. Accepted June 19, 1978. NMR spectra since two distinct peaks (6 -3.38, 3.78 ppm) caused by these protons are observed in CDCA vs. only one peak (6 -3.45 ppm) caused by the same protons in UDCA. T h e fingerprint region of the spectrum (6 1-2.5 ppm) and the relative position of the CIS methyl peak with respect to tha t of the doublet caused by the Cpl methy...
متن کاملSingle-step transesterification with simultaneous concentration and stable isotope analysis of fatty acid methyl esters by gas chromatography-combustion-isotope ratio mass spectrometry.
Gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS) is increasingly applied to food and metabolic studies for stable isotope analysis (δ(13) C), with the quantification of analyte concentration often obtained via a second alternative method. We describe a rapid direct transesterification of triacylglycerides (TAGs) for fatty acid methyl ester (FAME) analysis by GC-C-IRMS d...
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ژورنال
عنوان ژورنال: BUNSEKI KAGAKU
سال: 2014
ISSN: 0525-1931
DOI: 10.2116/bunsekikagaku.63.279